| Registro completo |
| Provedor de dados: |
OAK
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| País: |
Japan
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| Título: |
Freeze-dried Spermatozoa and Freeze-dried Sperm Injection into Oocytes
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| Autores: |
SUZUKI, Hiroshi
鈴木, 宏志
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| Data: |
2006
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| Ano: |
2006
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| Palavras-chave: |
Spermatozoa
Freeze-drying
ICSI
Preservation
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| Resumo: |
Attempts to freeze-dry spermatozoa are not new. Successful conception and full-term development using freeze-dried spermatozoa were reported in the cow and rabbit prior to 1960. However, these preliminary results have not been confirmed. Spermatozoa become defective in motility after freeze-drying and are unable to fertilize eggs both in vivo and in vitro. However, in 1998, freeze-drying of mammalian spermatozoa was demonstrated without loss of genetic or reproductive potential. Freeze-dried spermatozoa support normal development when injected directly into oocytes by means of an intracytoplasmic sperm injection (ICSI) technique. Recent investigations of freeze-dried sperm have focused on the factors affecting freeze-drying in spermatozoa in an effort to make it possible to store the male gamete at ambient temperature. The pressure level at primary dryied of freeze-drying spermatozoa, in addition to the components of the suspending solution, appears to be an important factor for long-term preservation of freeze-dried spermatozoa.
※J-stageにて公開
https://www.jstage.jst.go.jp/article/jmor/23/3/23_3_91/_article/-char/ja/
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| Idioma: |
Inglês
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| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/4189
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| Editor: |
日本卵子学会
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| Direitos: |
日本卵子学会
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